基于“言象意”思想的上海方塔园诗意空间营造研究

探析"言象意"思想与园林意境营造之间的联系,总结出立意、取象与尽意的园林意境空间营造方法。以方塔园为例,分析其在立意、取象与尽意等方面的营造手法,证实基于"言象意"思想的营造手法在诗意空间营造中的可行性与实用性,从中得到对现代诗意空间营造的启示:自然与生态精神的追求,传统文化的提取,现代资源的应用与诗性思维的参与。     

千岛湖森林康养基地发展路径探析 ——基于千岛湖龙川湾的调查研究

国家相关管理部门联合提出按照“环境优良、服务优质、管理完善、特色鲜明、效益明显”的要求建设一批国家森林康养基地。以浙江千岛湖龙川湾为例,分析其森林资源、地理区位、产业链、发展规模、社会合作、管理保障、三大效益等方面建设森林康养基地的优势,整合提升游憩观赏、研学文化、垂钓休闲、扶犁耕地、康养保健、疗养康复、健身拓展区域空间布局与发展举措,从康养体验、休闲体验、运动健身等方面增强千岛湖龙川湾森林康养基地功能,推进森林康养产业发展。     

基于森林健康理念的森林康养产业发展研究

阐述了森林健康理念和森林康养的基本概念,介绍了森林健康理念和森林康养的发展现状,分析了森林健康与森林康养产业之间的关系。结果表明:森林康养产业的理论基础就是基于森林健康理念继承发展来的,必然会促进森林健康产业的发展。运用森林健康理念指导森林康养产业发展是森林康养产业可持续发展的有效途径,森林健康理念在中国会不断完善和发展,也更有利于中国森林康养产业的发展。展望康养产业,会形成一种新的经济形态,成为国民福祉。     

右江流域水生态补偿机制研究初探

为保护右江流域生态环境,协调郁江乃至珠江上下游各方经济利益,更好的激励上游地区生态环境保护行为,通过分析右江流域水生态现状、存在问题、现有生态补偿政策环境及其在郁江流域、珠江流域中的地位和作用,利用外部性理论,分析提出了右江流域水生态补偿模式。通过对标对表有关生态保护规划和区划成果,立足百色市实际,提出了右江流域水生态补偿内容、补偿主客体、补偿标准和补偿方式,初步构建右江流域水生态补偿机制框架,并提出有针对性的补偿政策建议,为水生态补偿实施提供了参考。     

Role of TLR4-TRPC6 in LPS-induced NF-κB P65 expression and nuclear translocation in airway epithelial 16HBE cells

AIM: To investigate the role of Toll-like receptor 4 (TLR4) and transient receptor potential channel 6 (TRPC6) signaling pathway in lipopolysaccharide (LPS)-induced nuclear factor-κB (NF-κB) P65 expression and nuclear translocation in airway epithelial cells (16HBE) for supplementing the mechanism for airway inflammation. METHODS: After stimulating the 16HBE cells with LPS at 1 mg/L for 0, 0.5, 2, 6, 12 and 24 h, the expression of NF-κB P65 at mRNA and protein levels in the 16HBE cells were determined by RT-PCR and Western blot respectively, and the nuclear translocation of NF-κB P65 was detected by immunocytochemical staining method. The effects of TLR4 inhibitor CLI-095 at 5 μmol/L and TRPC6 agonist Hyp9 at 10 μmol/L on LPS (1 mg/L)-induced NF-κB P65 expression and nuclear translocation in the 16HBE cells were determined by RT-PCR, Western blot and immunocytochemical staining. RESULTS: LPS increased the mRNA and protein expression of NF-κB P65 and nuclear translocation in the 16HBE cells(P<0.05). TLR4 inhibitor CLI-095 reduced the mRNA and protein expression of NF-κB P65 and nuclear translocation induced by LPS, while Hyp9 enhanced the mRNA and protein expression of NF-κB P65 and nuclear translocation induced by LPS in the 16HBE cells(P<0.05). CONCLUSION: LPS induces the expression and nuclear translocation of NF-κB P65 in the 16HBE cells via TLR4-TRPC6 signaling pathway.     

基于温度植被干旱指数的曲靖市干旱监测研究

本研究利用MODIS归一化植被指数(NDVI)和地表温度(Ts)产品数据,构建Ts-NDVI特征空间,计算温度植被干旱指数(TVDI),分析评价云南曲靖市2010—2012年的干旱时空分布状况。结果表明,2010年曲靖市遭遇了非常严重的干旱灾害,受灾面积高达研究区总面积的80%以上,基于TVDI指数的旱情动态监测方法适合云南省曲靖干旱监测。本研究建立的温度植被干旱指数模型提高了干旱监测的实用性,在曲靖市干旱监测上具有很好的适应性,可以广泛应用到该地区干旱监测研究中,为曲靖的农业防灾减灾能力和可持续发展提供科学保障。     

高压雾化设备在现代农业中的应用

高压雾化设备指利用高压雾化主机将经过精密过滤处理的水,输送到造雾专用高压管网,最后到达造雾专用喷头喷出成雾的系统。高压雾化设备在现代农业中主要应用在设施农业降温、加湿、植保以及景观等方面。其中,设施温室降温时依据地域的气候条件、系统投资、后期维护费用、运行费用、降温效果以及对温室湿度的影响等各种因素,来综合考虑高压雾化与其他降温方式联合使用降温,以期达到最佳效果。根据工程项目使用高压雾化设备的经验总结了相关建议,以期为实际生产提供参考。     

Regulatory role of l-proline in fetal pig growth and intestinal epithelial cell proliferation

l-proline (Pro) is a precursor of ornithine, which is converted into polyamines via ornithine decarboxylase (ODC). Polyamines plays a key role in the proliferation of intestinal epithelial cells. The study investigated the effect of Pro on polyamine metabolism and cell proliferation on porcine enterocytes in vivo and in vitro. Twenty-four Huanjiang mini-pigs were randomly assigned into 1 of 3 groups and fed a basal diet that contained 0.77% alanine (Ala, iso-nitrogenous control), 1% Pro or 1% Pro + 0.0167% α-difluoromethylornithine (DFMO) from d 15 to 70 of gestation. The fetal body weight and number of fetuses per litter were determined, and the small and large intestines were obtained on d 70 ± 1.78 of gestation. The in vitro study was performed in intestinal porcine epithelial (IPEC-J2) cells cultured in Dulbecco''s modified Eagle medium-high glucose (DMEM-H) containing 0 μmol/L Pro, 400 μmol/L Pro, or 400 μmol/L Pro + 10 mmol/L DFMO for 4 d. The results showed that maternal dietary supplementation with 1% Pro increased fetal weight; the protein and DNA concentrations of the fetal small intestine; and mRNA levels for potassium voltage-gated channel, shaker-related subfamily, member 1 (Kv1.1) in the fetal small and large intestines (P < 0.05). Supplementing Pro to either gilts or IPEC-J2 cells increased ODC protein abundances and polyamine concentrations in the fetal intestines and IPEC-J2 cells (P < 0.05). In comparison with the Pro group, the combined administration of Pro and DFMO reduced the expression of ODC protein and spermine concentration in the fetal intestine, as well as the concentrations of putrescine, spermidine and spermine in IPEC-J2 cells (P < 0.05). Meanwhile, the percentage of cells in the S-phase and the mRNA levels of proto-oncogenes c-fos and c-myc were increased in response to Pro supplementation, whereas depletion of cellular polyamines with DFMO increased tumor protein p53 (p53) mRNA levels (P < 0.05). Taken together, dietary supplementation with Pro improved fetal pig growth and intestinal epithelial cell proliferation via enhancing polyamine synthesis.     

山东省果品产业高质量发展的意义及实现途径

果品产业高质量发展是兼顾经营者、生产者、消费者利益的更高层面的发展;推动山东果品产业高质量发展对于应对产业面临的挑战与压力、促进乡村振兴、落实新发展理念、增加农民收入等都具有十分重要的意义;山东果品产业发展水平具备了由追求高产量向追求高质量发展转变的客观条件;推动山东果品产业高质量发展,需要在充分发挥市场配置资源的决定性作用的同时,更好地发挥政府作用。     

Identification of an H1N1 subtype of swine influenza virus and serological analysis

To investigate the epizootic of swine influenza virus(SIV), 60 nasal swabs were collected from a clinical cases of pig farm in Tai'an City, Shandong Province of China in April 2017. SIV was isolated by inoculating into 10-day-old Special Pathogen Free embryonated eggs and the whole genome was sequenced. An H1N1 subtype SIV was isolated and designated as A/swine/Shandong/TA04/2017(H1N1). Phylogenetic analysis showed that apart from the polymerase A(PA) fragment belonging to the 2009 pandemic H1N1 branch, seven genome segments belonged to avian-like H1N1 influenza virus lineage. The cleavage site sequence of the hemagglutinin(HA) protein was PSIQSR↓G, which is a typical molecular biological characteristic. Five potential N-glycosylation sites(N14, N26, N277, N484 and N543) were found in the HA gene. To further investigate the epidemiology of SIV in this farm, the 995 serum samples were assessed with EAH1N1 2009 pandemic H1N1 and H3 N2 antigens. The results showed that the total positive rate was 65.43%. The positive rates of single virus infection detected by EAH1N1, 2009 pdmH1N1 and H3 N2 for serum HI(Hemagglutination inhibition) were 48.35, 30.85 and 7.47%, respectively. The results showed that SIV in Shandong Province has been reassorted in some segments and the SIV-positive rate was high on the SIV outbreak farm. These data provide evidence of an epizootic of SIV.